Evaluation of Serum IL-10, TNF-α and Albumin Levels among Male Type 2 Diabetes Mellitus Patients in a Tertiary Hospital in Nigeria
Ehiaghe, F.A.
Department of Immunology and Immunochemistry, Nnamdi Azikiwe University, Anambra State, Nigeria.
Ahmed, V.U.
Department of Clinical Chemistry, Nnamdi Azikiwe University, Anambra State, Nigeria.
Ehiaghe, J.I. *
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Benson Idahosa University, Benin City, Nigeria.
Ogalagu, R.O.
Department of Biochemistry, Faculty of Natural and Applied Sciences, Tansian University, Umunya, Nigeria.
Obu, S.I.
Centre for Communicable Disease Control and Research, Federal Medical Center, Asaba, Delta State, Nigeria.
Ize-Iyamu, J. A
Edo State Health Management Board, Benin City, Edo State, Nigeria.
Osakue, O.N.
Department of Clinical Chemistry, Nnamdi Azikiwe University, Anambra State, Nigeria.
Ogbodo, E.C.
Department of Clinical Chemistry, Nnamdi Azikiwe University, Anambra State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Type 2 diabetes (T2DM) is associated with a chronic inflammatory state and a disturbance in cytokine balance. This study aimed to evaluate the levels of TNF-α, IL-10, and albumin in the serum of males with type 2 diabetes compared to healthy individuals. A case-control study was conducted involving 60 participants (30 patients and 30 healthy individuals), and the samples were analyzed using ELISA techniques and the BCG method. The results showed a significant increase in TNF-α (53.97 ± 10.22 Vs 15.11 ± 2.82) and IL-10 (17.07 ± 1.42 Vs 4.12 ± 0.88) levels among the patients (P = <0.001), with no significant difference in albumin levels (p>0.05). The results indicate the presence of a simultaneous inflammatory and anti-inflammatory response, which underscores the importance of these cytokines as biomarkers in diabetes monitoring.
Keywords: Diabetes mellitus, inflammation, biomarkers, TNF-α, IL-10, serum albumin, male T2DM patients